Presenilin 1 Antibody (APS 11)

Presenilin 1 Monoclonal Antibody for ELISA, ICC, IF, IHC (P), IHC (PFA), IP, Western blot

>> See 7 other antibodies for PSEN1 (PS1, AD3, Alzheimer Disease 3, FAD, Homo Sapiens Clone CC44 Senilin 1, PSEN1, PSNL1, S182 Protein, Senilin 1)

Synonyms:

PS1, AD3, Alzheimer Disease 3, FAD, Homo Sapiens Clone CC44 Senilin 1, PSEN1, PSNL1, S182 Protein, Senilin 1

Entrez Gene ID:

(Hu) 5663, (Ms) 19164, (Rt) 29192

UniProt ID:

(Hu) P49768, (Ms) P49769, (Rt) P97887

Details

Host / Isotype:	Mouse / IgG1
Class:	Monoclonal
Type:	Antibody
Clone:	APS 11
Species Reactivity:	Human (Hu) Mouse (Ms) Rat (Rt) Non-human primate (Nhp)
Immunogen:	Synthetic peptide corresponding to residues C H(21) L S N T V R S Q N D N R E(34) of human PS1.

Ordering Information
Pierce Presenilin 1 Antibody (APS 11)

Product #

MA1-751

Size

200 µg

Price

$358.00

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Storage:	-20° C, Avoid Freeze/Thaw Cycles
Form:	200 ug protein G purified antibody (1mg/ml) in PBS containing 1 mg/ml BSA and 0.05% sodium azide.

Applications	Dilution *

Immunofluorescence (IF)	0.75 ug/ml
Western Blot (WB)	12 ug/ml
ELISA (ELISA)	4.5 ug/ml
Immunocytochemistry (ICC)	Assay dependent
Immunohistochemistry (Paraffin) (IHC (P))	0.75 ug/ml
Immunohistochemistry (PFA fixed) (IHC (PFA))	Assay Dependent
Immunoprecipitation (IP)	Assay Dependent
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Product Specific Information

MA1-751 detects presenilin 1 protein (PS1) from mouse, rat, human, and nonhuman primate samples. No cross-reactivity is seen with presenilin 2.

MA1-751 has successfully been used immunofluorescence, immunocytochemistry, immunohistochemistry, Western blot , and ELISA procedures. By Western blot, this antibody detects an ~28 kDa protein representing PS1 N-terminus cleavage product from ST15 cell lysate transfected with human PS1. In formalin-fixed, paraffin embedded sections of human brain, this antibody showed strong staining of both the plaque core and dystrophic neurites.

The MA1-751 immunogen is a synthetic peptide corresponding to residues C H(21) L S N T V R S Q N D N R E(34) of human PS1.

General Information

Familial Alzheimer’s disease is often characterized by an early (<60 years of age) and rapid deterioration of the central nervous system. The symptoms are caused by the abnormal buildup of senile plaques composed of the 42 residue amyloid-beta peptide. This peptide is the result of the amyloid precursor protein being cleaved by the presenilin-gamma-secretase complex. Recent studies have linked mutations in presenilin 1 (PS1) and presenilin 2 (PS2) to a rapid increase in plaque accumulation.

PS1 and PS2 are integral membrane proteins that contain 6-8 transmembrane domains and are predominantly localized within the endoplasmic reticulum and Golgi of neurons within the brain. Within the transmembrane regions of PS1 and PS2 there is over 60% homology, with the largest divergence found at the N-terminus and large loop region.

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Presenilin 1 Antibody (MA1-751) in IHC

Immunohistochemistry with anti-Presenilin 1 Monoclonal Antibody [APS 11] (MA1-751)

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Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing Presenilin 1 (MA1-751) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

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Presenilin 1 Antibody (MA1-751) in IHC

Immunohistochemistry with anti-Presenilin 1 Monoclonal Antibody [APS 11] (MA1-751)

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Immunohistochemistry was performed on normal biopsies of deparaffinized Human liver tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Presenilin 1 (MA1-751) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

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Presenilin 1 Antibody (MA1-751) in ICC

Immunocytochemistry with anti-Presenilin 1 Monoclonal Antibody [APS 11] (MA1-751)

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Immunocytochemical staining of PS1 in mouse fibroblasts using MA1-751.

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PubMed References:

ELISA (1)
ICC (1)
WB (1)

ELISA
	Species / Dilution	Summary
	Hu / 3 ug/ml	MA1-751 was used in ELISA, immunocytochemistry and western blot to study the presenilin 1 and presenilin 2 expression and processing by monoclonal antibodies J Neurosci Res. 1999 May 15;56(4):405-19. "Analysis of presenilin 1 and presenilin 2 expression and processing by newly developed monoclonal antibodies." Author(s): Diehlmann A, Ida N, Weggen S, Grünberg J, Haass C, Masters CL, Bayer TA, Beyreuther K Number of Citations: 3 (See PubMed article )

Immunocytochemistry
	Species / Dilution	Summary
	Hu / 0.05 ug/ul	MA1-751 was used in ELISA, immunocytochemistry and western blot to study the presenilin 1 and presenilin 2 expression and processing by monoclonal antibodies J Neurosci Res. 1999 May 15;56(4):405-19. "Analysis of presenilin 1 and presenilin 2 expression and processing by newly developed monoclonal antibodies." Author(s): Diehlmann A, Ida N, Weggen S, Grünberg J, Haass C, Masters CL, Bayer TA, Beyreuther K Number of Citations: 3 (See PubMed article )

Western Blot
	Species / Dilution	Summary
	Hu / 4 ug/ml	MA1-751 was used in ELISA, immunocytochemistry and western blot to study the presenilin 1 and presenilin 2 expression and processing by monoclonal antibodies J Neurosci Res. 1999 May 15;56(4):405-19. "Analysis of presenilin 1 and presenilin 2 expression and processing by newly developed monoclonal antibodies." Author(s): Diehlmann A, Ida N, Weggen S, Grünberg J, Haass C, Masters CL, Bayer TA, Beyreuther K Number of Citations: 3 (See PubMed article )

(This product is for In Vitro experimental use only. Not for resale without express authorization.)