Catenin beta Antibody (9F2)

Catenin beta Monoclonal Antibody for IF, IP, Western blot

>> See 24 other antibodies for CTNNB1 (Cadherin associated protein, Catenin beta 1, CATNB, CTNNB)
Synonyms:
Cadherin associated protein, Catenin beta 1, CATNB, CTNNB
Entrez Gene ID:
(Hu) 1499, (Ms) 12387
UniProt ID:
(Hu) P35222, (Ms) Q02248
Details
Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Antibody
Clone: 9F2
Species Reactivity: Human (Hu) Mouse (Ms)
Immunogen: 120 kDa MBP/beta catenin fusion protein purified on amylose column.
Ordering Information
Pierce Catenin beta Antibody (9F2)
Product #
MA1-2001
Size
100 µg
Price
$239.00
Purchase
Add Catenin beta Antibody (9F2) to your cart.
Storage: -20° C, Avoid Freeze/Thaw Cycles
Form: 100 ug of Protein G purified IgG (1 mg/ml) antibody in PBS with 1 mg/ml BSA and 0.05% sodium azide.


Applications Dilution *
Western Blot (WB) Assay dependent
Immunoprecipitation (IP) 1 ug/ml
Immunofluorescence (IF) 10 ug/ml
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
MA1-2001 detects beta catenin from human samples.

MA1-2001 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~94 kDa protein representing beta catenin from A431 cell extract. MA1-2001 can also be used in immunoprecipitation and immunofluorescence procedures.

The MA1-2001 immunogen is a 120 kDa MBP/ beta catenin fusion protein purified via an amylose column.
General Information
The Catenins (alpha, beta and gamma) are ubiquitously expressed cytoplasmic proteins, which are associated with E-cadherin. Beta Cadherin can also bind to N-cadherin and co-immunoprecipitates with APC. Cadherin/Catenin complexes are linked to the cytoskeleton via a direct association between alpha Actinin and alpha Catenin.
Product Images
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  • Catenin beta Antibody (MA1-2001) in IF
    Immunofluorescence with anti-Catenin beta Monoclonal Antibody [9F2] (MA1-2001)

    Immunofluorescence with anti-Catenin beta Monoclonal Antibody [9F2] (MA1-2001)

    View Product Details >>
    Immunofluorescent analysis of Beta-Catenin using Beta-Catenin Monoclonal antibody (9F2) (Product# MA1-2001) shows staining in HeLa cells. Beta-Catenin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Beta-Catenin (Product# MA1-2001) at a dilution of 1:20 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
     
  • Catenin beta Antibody (MA1-2001) in IF
    Immunofluorescence with anti-Catenin beta Monoclonal Antibody [9F2] (MA1-2001)

    Immunofluorescence with anti-Catenin beta Monoclonal Antibody [9F2] (MA1-2001)

    View Product Details >>
    Immunofluorescent analysis of Beta-Catenin using Beta-Catenin Monoclonal antibody (9F2) (Product# MA1-2001) shows staining in U251 glioma cells. Beta-Catenin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Beta-Catenin (Product# MA1-2001) at a dilution of 1:20 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
     
 
 
PubMed References:
Immunocytochemistry
  Species / Dilution Summary
  Hu / Not Cited
 MA1-2001 was used in immunocytochemistry and western blot to investigate the interaction of plakoglobin with N-cadherin and alpha catenin
J Biol Chem. 1995 Aug 25;270(34):20201-6.
"Identification of plakoglobin domains required for association with N-cadherin and alpha-catenin."
Author(s): Sacco PA, McGranahan TM, Wheelock MJ, Johnson KR
Number of Citations: 26
(See PubMed article )
Immunoprecipitation
  Species / Dilution Summary
  Hu / Not Cited
 MA1-2001 was used in immunoprecipitation and western blot to investigate the interaction between alpha-actinin and N-cadherin/catenin complex
J Cell Biol. 1995 Jul;130(1):67-77.
"Interaction of alpha-actinin with the cadherin/catenin cell-cell adhesion complex via alpha-catenin."
Author(s): Knudsen KA, Soler AP, Johnson KR, Wheelock MJ
Number of Citations: 81
(See PubMed article )
Western Blot
  Species / Dilution Summary
  Hu / Not Cited
 MA1-2001 was used in immunocytochemistry and western blot to investigate the interaction of plakoglobin with N-cadherin and alpha catenin
J Biol Chem. 1995 Aug 25;270(34):20201-6.
"Identification of plakoglobin domains required for association with N-cadherin and alpha-catenin."
Author(s): Sacco PA, McGranahan TM, Wheelock MJ, Johnson KR
Number of Citations: 26
(See PubMed article )
  Hu / Not Cited
 MA1-2001 was used in immunoprecipitation and western blot to investigate the interaction between alpha-actinin and N-cadherin/catenin complex
J Cell Biol. 1995 Jul;130(1):67-77.
"Interaction of alpha-actinin with the cadherin/catenin cell-cell adhesion complex via alpha-catenin."
Author(s): Knudsen KA, Soler AP, Johnson KR, Wheelock MJ
Number of Citations: 81
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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