Neurofibromin Antibody (McNFn27b)

Neurofibromin Monoclonal Antibody for IF, IHC, Western blot

>> See 4 other antibodies for NF1 (NF1, WSS, NFNS, VRNF)
Synonyms:
NF1, WSS, NFNS, VRNF
Entrez Gene ID:
(Hu) 4763, (Ms) 18015, (Rt) 24592
UniProt ID:
(Hu) P21359, (Ms) Q04690, (Rt) P97526
Details
Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Antibody
Clone: McNFn27b
Species Reactivity: Human (Hu) Mouse (Ms) Rat (Rt)
Immunogen: Synthetic peptide corresponding to the N-terminal residues 27-41 of human neurofibromin.
Ordering Information
Pierce Neurofibromin Antibody (McNFn27b)
Product #
MA1-085
Size
200 µg
Price
$299.00
Purchase
Add Neurofibromin Antibody (McNFn27b) to your cart.
Storage: -20° C, Avoid Freeze/Thaw Cycles
Form: 200 ug of protein G purified IgG antibody (1 mg/ml) in PBS with 1 mg/ml BSA and 0.05% sodium azide.


Applications Dilution *
Western Blot (WB) 10 ug/mL
Immunohistochemistry (IHC) 5-10 ug/mL
Immunofluorescence (IF) 1:20
Immunofluorescence (IF) 1:10-1:100
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
MA1-085 detects neurofibromin in human, rat, and mouse cells.

MA1-085 has been successfully used in Western blot, and immunohistochemistry procedures. By Western blot, this antibody detects a ~240-260 kDa protein.

The MA1-085 immunogen is a synthetic peptide corresponding to the N-terminal residues 27-41 of human neurofibromin.
General Information
Neurofibromin is a member of the GTPase-activating (GAP) class of proteins. These proteins enhance the GTPase activity of signaling G-Proteins like Ras. The NF1 gene is also classified as a tumor suppressor gene, and many studies have shown that mutations at the NF1 locus produce neurofibromas, rhabdomyosarcomas, leimyosarcomas, mental retardation and memory deficiency. Neurofibromin has also been associated with the intermediated filaments of the cytoskeleton. NF1 is expressed at its highest levels during periods of desmosome formation and mutations of NF1 produce variable cell sizes and morphologies.
Product Images
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  • Neurofibromin Antibody (MA1-085) in IF
    Immunofluorescence with anti-Neurofibromin Monoclonal Antibody [McNFn27b] (MA1-085)

    Immunofluorescence with anti-Neurofibromin Monoclonal Antibody [McNFn27b] (MA1-085)

    View Product Details >>
    Immunofluorescent analysis of Neurofibromin in Hela cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Neurofibromin monoclonal antibody (Product # MA1-085) at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). Neurofibromin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
     
  • Neurofibromin Antibody (MA1-085) in IF
    Immunofluorescence with anti-Neurofibromin Monoclonal Antibody [McNFn27b] (MA1-085)

    Immunofluorescence with anti-Neurofibromin Monoclonal Antibody [McNFn27b] (MA1-085)

    View Product Details >>
    Immunofluorescent analysis of Neurofibromin in NIH-3T3 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Neurofibromin monoclonal antibody (Product # MA1-085) at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). Neurofibromin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
     
  • Neurofibromin Antibody (MA1-085) in IF
    Immunofluorescence with anti-Neurofibromin Monoclonal Antibody [McNFn27b] (MA1-085)

    Immunofluorescence with anti-Neurofibromin Monoclonal Antibody [McNFn27b] (MA1-085)

    View Product Details >>
    Immunofluorescent analysis of Neurofibromin in U251 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Neurofibromin monoclonal antibody (Product # MA1-085) at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). Neurofibromin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
     
  • Neurofibromin Antibody (MA1-085) in IHC
    Immunohistochemistry with anti-Neurofibromin Monoclonal Antibody [McNFn27b] (MA1-085)

    Immunohistochemistry with anti-Neurofibromin Monoclonal Antibody [McNFn27b] (MA1-085)

    View Product Details >>
    Immunohistochemistry was performed on cancer biopsies of deparaffinized Human breast carcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and probed with a Neurofibromin monoclonal antibody (Product # MA1-085) at a dilution of 1:20 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
     
  • Neurofibromin Antibody (MA1-085) in IHC
    Immunohistochemistry with anti-Neurofibromin Monoclonal Antibody [McNFn27b] (MA1-085)

    Immunohistochemistry with anti-Neurofibromin Monoclonal Antibody [McNFn27b] (MA1-085)

    View Product Details >>
    Immunohistochemistry was performed on normal biopsies of deparaffinized Human stomach tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and probed with a Neurofibromin monoclonal antibody (Product # MA1-085) at a dilution of 1:20 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
     
 
 
References:
Am. J. Pathology 158(2):501-513, 2001.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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