TNF Alpha Antibody (2TNF-H34A)
TNF Alpha Monoclonal Antibody for ELISA, Western blot
Synonyms:
APC1 protein; TNF superfamily, member 2; TNF, macrophage-derived; TNF, monocyte-derived; TNF-a; cachectin; tumor necrosis factor alpha; tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha
Entrez Gene ID:
UniProt ID:
(Hu) P01375
| Details | |
| Host / Isotype: | Mouse / IgG1, kappa |
| Class: | Monoclonal |
| Type: | Antibody |
| Clone: | 2TNF-H34A |
| Species Reactivity: | Human (Hu) |
| Immunogen: | Recombinant human TNF alpha |
| Ordering Information | ||||
| Pierce TNF Alpha Antibody (2TNF-H34A) |
| Storage: | -20°C |
| Form: | 500 µg of purified antibody from ascites fluid by Protein G affinity chromatography; in preservative and carrier-free PBS |
| Applications | Dilution * |
| ELISA (ELISA) | Assay Dependent |
| Western Blot (WB) | Assay Dependent |
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* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
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| Product Specific Information |
| M303 targets TNF Alpha in ELISA, and WB applications and shows reactivity with Human samples. The M303 antibody (clone 2TNF-H34A) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody M302B (biotinylated conjugate of clone 2TNG-H33). The M303 immunogen is recombinant human TNF alpha. M303 detects TNF Alpha which has a predicted molecular weight of approximately 26 kDa. The M303 TNFa antibody (clone 2TNF-H34A) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody M302B (biotinylated conjugate of clone 2TNF-H33) Typical dilutions for sandwich ELISA include 1 µg/ml for coating and 0.125 - 0.25 µg/ml for detection. Antibody M303 (clone 2TNF-H34A) and biotinylated antibody M302B (clone 2TNF-H33) have successfully been used in combination with recombinant TNFa protein STNFA in ELISA applications. |
| General Information |
| This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. |
| PubMed References: |
| ELISA | ||
| Species / Dilution | Summary | |
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Hu / Not Cited |
M303 was used in ELISA to assess the interferon-alpha production in peripheral blood mononuclear cell
J Exp Med. 1997 Jun 2;185(11):1987-95.
"Regulation of interleukin-12 by complement receptor 3 signaling." Author(s): Marth T, Kelsall BL Number of Citations: 42 (See PubMed article  )
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Hu / 4 mg/ml |
M303 was used in ELISA to study the role of inteferon gamma in EBV-transformed B cells
Viral Immunol. 2002;15(1):213-25.
"Interferon--gamma control of EBV-transformed B cells: a role for CD8+ T cells that poorly kill EBV-infected cells." Author(s): Shi Y, Lutz CT Number of Citations: 1 (See PubMed article )
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Hu / 2 ug/ml |
M303 was used in ELISA to study the role of type 1 cytokine in chronic hepatosplenic schistosomiasis
Infect Immun. 2002 Nov;70(11):5903-12.
"Enhanced interleukin-12 and CD40 ligand activities but reduced Staphylococcus aureus Cowan 1-induced responses suggest a generalized and progressively impaired type 1 cytokine pattern for human schistosomiasis." Author(s): Montenegro SM, Abath FG, Domingues AL, Melo WG, Morais CN, Coutinho EM, Mahanty S, Wynn TA Number of Citations: 0 (See PubMed article )
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Hu / Not Cited |
M303 was used in ELISA to investigate the role of plasmacytoid dendritic cells (pDCs) in adaptive immunity
Blood. 2003 May 1;101(9):3520-6.
"Activation of influenza virus-specific CD4+ and CD8+ T cells: a new role for plasmacytoid dendritic cells in adaptive immunity." Author(s): Fonteneau JF, Gilliet M, Larsson M, Dasilva I, Münz C, Liu YJ, Bhardwaj N Number of Citations: 1 (See PubMed article )
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Hu / Not Cited |
M303 was used in ELISA to investigate the augmented production of Th1-type cytokines in HCV-infected cryoglobulinemic patients
J Hepatol. 2003 Feb;38(2):230-6.
"Enhanced monocyte Th1 cytokine production in HCV-infected cryoglobulinemic patients." Author(s): Loffreda S, Muratori P, Muratori L, Mele L, Bianchi FB, Lenzi M Number of Citations: 2 (See PubMed article )
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Hu / Not Cited |
M303 was used in ELISA to investigate the generation of three-dimensional pannus-like tissues in vitro from single cell suspensions of synovial fluid cells from arthritis patients
Rheumatol Int. 2004 Mar;24(2):71-6.
"Generation of three-dimensional pannus-like tissues in vitro from single cell suspensions of synovial fluid cells from arthritis patients." Author(s): Solomon S, Masilamani M, Mohanty S, Schwab JE, Boneberg EM, Illges H Number of Citations: 1 (See PubMed article )
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Hu / Not Cited |
M303 was used in ELISA to study the effect of etanercept treatment on proinflammatory cytokine-secreting peripheral blood mononuclear cells in rheumatoid arthritic patients
Rheumatology (Oxford). 2004 Aug;43(8):960-4.
"Long-term treatment with etanercept significantly reduces the number of proinflammatory cytokine-secreting peripheral blood mononuclear cells in patients with rheumatoid arthritis." Author(s): Schotte H, Schlüter B, Willeke P, Mickholz E, Schorat MA, Domschke W, Gaubitz M Number of Citations: 1 (See PubMed article )
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Hu / Not Cited |
M303 was used in ELISA to study the effect of genotypes on the innate response against the candidate adjuvants RC529 and monophosphoryl lipid A
Vaccine. 2004 Mar 29;22(11-12):1515-23.
"Host factors impacting the innate response in humans to the candidate adjuvants RC529 and monophosphoryl lipid A." Author(s): Tiberio L, Fletcher L, Eldridge JH, Duncan DD Number of Citations: 1 (See PubMed article )
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Hu / Not Cited |
M303 was used in ELISA to characterize the thalidomide as tumor necross factor-alpha inhibitors and its possible therapeutic effects on neurodegenerative diseases
Acta Neurobiol Exp (Wars). 2004;64(1):1-9.
"Thalidomide-based TNF-alpha inhibitors for neurodegenerative diseases." Author(s): Greig NH, Giordano T, Zhu X, Yu QS, Perry TA, Holloway HW, Brossi A, Rogers JT, Sambamurti K, Lahiri DK Number of Citations: 1 (See PubMed article )
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Hu / 300 ng/ml |
M303 was used in ELISA to investigate the role of immunostimulant RU41740 from Klebsiella pneumoniae for innate and adaptive immune responses in human cells from whole blood
Int Immunopharmacol. 2006 Apr;6(4):635-46.
"The immunostimulant RU41740 from Klebsiella pneumoniae activates human cells in whole blood to potentially stimulate innate and adaptive immune responses." Author(s): Pedraza-Sánchez S, González-Hernández Y, Escobar-Gutiérrez A, Ramachandra L Number of Citations: 1 (See PubMed article )
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Hu / Not Cited |
M303 was used in ELISA to study the role of sesquiterpene lactones in human monocyte-derived macrophage responses
Apoptosis. 2007 Jan;12(1):141-53.
"Sesquiterpene lactones induce distinct forms of cell death that modulate human monocyte-derived macrophage responses." Author(s): López-Antón N, Hermann C, Murillo R, Merfort I, Wanner G, Vollmar AM, Dirsch VM Number of Citations: 1 (See PubMed article )
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Hu / 1:5000 |
M303 was used in ELISA to investigate an immobilization method for efficient and highly sensitive sandwich ELISA system with smaller amounts of ligand antibodies
J Biotechnol. 2009 Jun 15;142(2):135-41.
"Efficient immobilization of a ligand antibody with high antigen-binding activity by use of a polystyrene-binding peptide and an intelligent microtiter plate." Author(s): Kumada Y, Hamasaki K, Shiritani Y, Ohse T, Kishimoto M Number of Citations: 0 (See PubMed article )
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Hu / Not Cited |
M303 was used in ELISA to investigate the mechanism of human T cell regulation by anandamide
PLoS One. 2010 Jan 14;5(1):e8688.
"Anandamide suppresses proliferation and cytokine release from primary human T-lymphocytes mainly via CB2 receptors." Author(s): Cencioni MT, Chiurchiù V, Catanzaro G, Borsellino G, Bernardi G, Battistini L, Maccarrone M Number of Citations: 9 (See PubMed article )
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Hu / Not Cited |
M303 was used in ELISA to study the temporal cytokine profiles in chronic fatigue syndrome with and without fibromyalgia during sleep
Clin Vaccine Immunol. 2010 Apr;17(4):582-7.
"Cytokines across the night in chronic fatigue syndrome with and without fibromyalgia." Author(s): Nakamura T, Schwander SK, Donnelly R, Ortega F, Togo F, Broderick G, Yamamoto Y, Cherniack NS, Rapoport D, Natelson BH Number of Citations: 1 (See PubMed article )
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(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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TNF Alpha Antibodies > TNF Alpha Monoclonal Antibody M303
