CD25 Antibody (2R12)
CD25 Monoclonal Antibody for FACS, IHC, IP, Western blot
Synonyms:
IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA; TAC antigen; interleukin-2 receptor subunit alpha; p55
Entrez Gene ID:
UniProt ID:
(Hu) P01589
| Details | |
| Host / Isotype: | Mouse / IgG1, kappa |
| Class: | Monoclonal |
| Type: | Antibody |
| Clone: | 2R12 |
| Species Reactivity: | Human (Hu) |
| Immunogen: | p55 alpha chain of the human IL-2 receptor |
| Ordering Information | ||||
| Pierce CD25 Antibody (2R12) |
| Storage: | 4° C |
| Form: | 100 tests of purified IgG by Protein G affinity chromatography from mouse ascites fluid in PBS with 0.5% BSA and 0.1% sodium azide |
| Applications | Dilution * |
| Flow Cytometry (FACS) | Assay Dependent |
| Immunoprecipitation (IP) | Assay Dependent |
| Immunohistochemistry (IHC) | Assay Dependent |
| Western Blot (WB) | Assay Dependent |
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* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
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| Product Specific Information |
| M10AR targets CD25 in FACS, IHC, IP, and WB applications and shows reactivity with Human samples. The M10AR immunogen is p55 alpha chain of the human IL-2 receptor. M10AR detects CD25 which has a predicted molecular weight of approximately 28 kDa. |
| General Information |
| The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency. |
| PubMed References: |
| Blocking Assay | ||
| Species / Dilution | Summary | |
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Hu / 1:300 |
M10AR was used in blocking/activating experiment, flow cytometry and immunoprecipitation to investigate the functional and structural properties of interleukin 2 receptors on activated B cells
J Immunol. 1985 Apr;134(4):2387-92.
"Structural and functional characterization of IL 2 receptors on activated human B cells." Author(s): Boyd AW, Fisher DC, Fox DA, Schlossman SF, Nadler LM Number of Citations: 9 (See PubMed article  )
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| Flow Cytometry | ||
| Species / Dilution | Summary | |
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Hu / Not Cited |
M10AR was used in flow cytometry to investigate the granular lymphocyte expansions with specific monoclonal antibodies
Blood. 1992 Oct 1;80(7):1765-73.
"Heterogeneous immunophenotype of granular lymphocyte expansions: differential expression of the CD8 alpha and CD8 beta chains." Author(s): de Totero D, Tazzari PL, DiSanto JP, di Celle PF, Raspadori D, Conte R, Gobbi M, Ferrara GB, Flomenberg N, Lauria F Number of Citations: 3 (See PubMed article )
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Hu / 1:100-1:200 |
M10AR was used in blocking/activating experiment, flow cytometry and immunoprecipitation to investigate the functional and structural properties of interleukin 2 receptors on activated B cells
J Immunol. 1985 Apr;134(4):2387-92.
"Structural and functional characterization of IL 2 receptors on activated human B cells." Author(s): Boyd AW, Fisher DC, Fox DA, Schlossman SF, Nadler LM Number of Citations: 9 (See PubMed article )
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| Immunoprecipitation | ||
| Species / Dilution | Summary | |
|
Hu / Not Cited |
M10AR was used in blocking/activating experiment, flow cytometry and immunoprecipitation to investigate the functional and structural properties of interleukin 2 receptors on activated B cells
J Immunol. 1985 Apr;134(4):2387-92.
"Structural and functional characterization of IL 2 receptors on activated human B cells." Author(s): Boyd AW, Fisher DC, Fox DA, Schlossman SF, Nadler LM Number of Citations: 9 (See PubMed article )
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(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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CD25 Antibodies > CD25 Monoclonal Antibody M10AR
