Xenopus laevis (Xl) Human (Hu) Mouse (Ms) Rat (Rt)
Mitogen-activated protein kinase 8
Pierce MAPK8 Antibody
Western Blot (WB)
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
82381 targets MAPK8 in WB applications and shows reactivity with Human, Murine, Rat, and Xenopus laevis samples.
The 82381 immunogen is mitogen-activated protein kinase 8.
82381 detects MAPK8 which has a predicted molecular weight of approximately 46 kDa.
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.
MAPK8 Antibody (82381) in WB
Western Blot with anti-MAPK8 Polyclonal Antibody (82381)
A549 cells were lysed 72 hours after transfection. Cells were transfected with Transfection Reagent alone (Lane 1), 100nM ON-TARGETplus siCONTROL Non-Targeting Pool (Lane 2), or 100nM ON-TARGETplus MAPK8 siRNA (Lane 3). Western blot data for Cyclophilin B is included as a control for equal protein loading.