* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
82319 targets BCL2 in WB applications and shows reactivity with Human samples.
The 82319 immunogen is b-cell lymphoma protein 2.
82319 detects BCL2 which has a predicted molecular weight of approximately 25 kDa.
General Information
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends.
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BCL2 Antibody (82319) in WB
Western Blot with anti-BCL2 Monoclonal Antibody (82319)
HeLa cells were lysed 72 hours after transfection. Cells were transfected with Transfection Reagent alone (Lane 1), 100nM ON-TARGETplus siCONTROL Non-Targeting Pool (Lane 2), or 100nM ON-TARGETplus BCL2 siRNA (Lane 3). Western blot data for GAPDH is included as a control for equal protein loading.