100 µl of Protein G purified antibody (1 mg/ml) in PBS, pH 7.4.
2 - 10 µg/ml
2 - 10 µg/ml
Western Blot (WB)
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
82314 targets ATM in Western blot, immunocytochemistry, and immunofluorescence applications and shows reactivity with human, mouse, monkey, and rat samples. ATM has a predicted molecular weight of approximately 370 kDa.
The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. Two transcript variants encoding different isoforms have been found for this gene.
ATM Antibody (82314) in WB
Western Blot with anti-ATM Monoclonal Antibody [5C2] (82314)
HeLa cells were lysed 72 hours after transfection. Cells were transfected with Transfection Reagent alone (Lane 1), 100nM ON-TARGETplus siCONTROL Non-Targeting Pool (Lane 2), or 100nM ONTARGETplus ATM siRNA (Lane 3). Western blot data for Lamin A/C is included as a control for equal protein loading.