* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
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Product Specific Information
82310 targets AKT1 in WB applications and shows reactivity with Human, Murine, and Rat samples.
The 82310 immunogen is v-akt-1, Protein kinase B.
82310 detects AKT1 which has a predicted molecular weight of approximately 60 kDa.
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.
AKT1 Antibody (82310) in WB
Western Blot with anti-AKT1 Monoclonal Antibody (82310)
A549 cells were lysed 72 hours after transfection. Cells were transfected with Transfection Reagent alone (Lane 1), 100nM ON-TARGETplus siCONTROL Non-Targeting Pool (Lane 2), or 100nM ON-TARGETplus AKT1 siRNA (Lane 3). Western blot data for GAPDH is included as a control for equal protein loading.